This Mia40-induced folding had been suggested to help proteins to move into the intermembrane space. Many of these proteins have the ability to form disulfide bonds within their structures, which help the proteins to fold and assemble correctly, but they only acquire these bonds once they have entered the intermembrane space.Īn enzyme called Mia40 sits inside the intermembrane space and helps other proteins to fold correctly. Intermembrane space proteins are made in the cytosol before being transported into the intermembrane space through pores in the mitochondrion’s outer membrane. Each mitochondrion is surrounded by an inner and an outer membrane, which are separated by an intermembrane space that contains a host of molecules, including proteins. Human, yeast and other eukaryotic cells contain compartments called mitochondria that perform several vital tasks, including supplying the cell with energy. Our results indicate that Mia40 predominantly serves as a trans-site receptor of mitochondria that binds incoming proteins via hydrophobic interactions thereby mediating protein translocation across the outer membrane by a ‘holding trap’ rather than a ‘folding trap’ mechanism. An oxidase-deficient Mia40 mutant is inviable, but can be partially rescued by the addition of the chemical oxidant diamide. Thereby we show that the substrate-binding domain of Mia40 is both necessary and sufficient to promote protein import, indicating that trapping by Mia40 drives protein translocation. In this study, we generated yeast mutants to dissect both Mia40 activities genetically and biochemically. Mia40 consists of two functional elements: an N-terminal cysteine-proline-cysteine motif conferring substrate oxidation, and a C-terminal hydrophobic pocket for substrate binding. The conserved IMS protein Mia40 is essential for the oxidation and import of these proteins. (new proteins of the mitochondrial IMS contain conserved cysteines that are oxidized to disulfide bonds during their import. Protected override void OnCreateControl() Private void SetMainButton(ActionButtonInfo abi)įoreach(ActionButtonInfo abi in _actionButtons)ītnActions.DropDownOpening += OnbtnActions_DropDownOpening Int marginY = ( + ) ītnMainAction.Width = this.Width - _splitButtonWidth - marginX ītnActions.Width = _splitButtonWidth - marginX - 1 ītnMainAction.Height = this.Height - marginY ītnActions.Height = this.Height - marginY If(btnActions is null || btnMainAction is null) Private void ResizeButtons(bool suspend = true) Tdd.DefaultDropDownDirection = ToolStripDropDownDirection.BelowLeft ToolStripDropDownMenu tdd = btnActions.DropDown as ToolStripDropDownMenu ![]() Private void OnbtnActions_DropDownOpening(object sender, EventArgs e) If(e.ClickedItem != null & !String.IsNullOrEmpty(e.ClickedItem.Text))ĪctionButtonInfo abi = e.ClickedItem.Tag as ActionButtonInfo Private void OnActions_DropDownItemClicked(object sender, ToolStripItemClickedEventArgs e) ![]() TextImageRelation = TextImageRelation.ImageBeforeText, Width = btnMainAction.Width + btnActions.Width, Private void AddActionButtonImpl(ActionButtonInfo abi) / The actual implementation that adds a button to the button list Public new ToolStripLayoutStyle LayoutStyle1 => ToolStripLayoutStyle.Flow Public new ToolStripLayoutStyle LayoutStyle => _layoutStyle Public new ToolStripGripStyle GripStyle => ToolStripGripStyle.Hidden Public ToolStripDropDownDirection DropDownDirection ![]() / Gets or sets the drop down direction. Private ToolStripLayoutStyle _layoutStyle = ToolStripLayoutStyle.Flow Private List _actionButtons = new List() / Implements a drop button using only standard winform controls The button will make a once clicked drop item the main item, this can be changed in the OnActions_DropDownItemClicked() method There are a few minor layout issues like the alignment of the dropdown items, but nothing really serious. ![]() And it can be designed in the winform editor. So I have come up with a custom control that is based on a toolstrip and thus fully customizable with text and images, having its own click event for each button/action. (separatorPen, lineX, lineYFrom, lineX, lineYTo) MenuButton class: public class MenuButton : Button Button have down arrow right side of it and you can set menu of it from designer:
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